ChromeQ: optogenetic activation with reduced proton and calcium currents
Publication [Cho YK, Park D*, Yang A*, Chen F, Chuong AS, Klapoetke NC, Boyden ES (2019) Multidimensional screening yields channelrhodopsin variants having improved photocurrent and order-of-magnitude reductions in calcium and proton currents, Journal of Biological Chemistry 294(11):3806-3821. (*, equal contribution)]
Plasmids, plasmid maps, and plasmid preparation protocols, as well as viruses (AAV, lentivirus), available at [Addgene]
Viruses (AAV, lentivirus) available at [UNC vector core]
Viruses (AAV, lentivirus) available at [U. Penn vector core]
Contact [Prof. Ed Boyden] with questions, comments, or to check for updates.