Synthetic Neurobiology Group

Ed Boyden, Ph.D., Principal Investigator

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Resources

Tools for mapping the molecular structure of the brain

  • Expansion microscopy: physical magnification with nanoscale precision

  • Pumby: programmable, modular RNA binding proteins

Tools for recording high-speed brain dynamics

  • SiRIs: spatial multiplexing of fluorescent reporters for imaging signaling network dynamics

  • SomaGCaMP: soma-targeted calcium indicators for precision neural population imaging

  • SomArchon: voltage indicator for population neural activity imaging

  • Archon1: well-localized, high-sensitivity, photostable fluorescent voltage indicator

  • Lightfield microscopy: fast 3-D neural imaging

  • Autopatching: robotic patch clamp in live brain

Optogenetics: molecules enabling neural control by light

  • ChromeQ: optogenetic activation with reduced proton and calcium currents

  • soCoChR: soma-targeted, high-amplitude channelrhodopsin

  • Jaws: red light-drivable halorhodopsin

  • Chrimson: red light-drivable channelrhodopsin

  • Chronos: high-speed, light-sensitive, channelrhodopsin

  • CoChR: large-current channelrhodopsin

  • Lumitoxins: light-actuated tethered toxin architecture

  • ArchT: light-sensitive archaerhodopsin

  • Arch: archaerhodopsin (light-driven proton pump)

  • Mac: light-driven proton pump of L. maculans

  • ChR2-2A-Halo: channelrhodopsin-halorhodopsin gene fusions

  • Halo/NpHR: halorhodopsin (light-driven chloride pump)

  • ChR2: channelrhodopsin-2 (light-driven cation channel)

  • Glial optogenetic tools

  • Accessory plasmids for optogenetics

  • Lentivirus production for high-titer, cell-specific, in vivo neural labeling

Optogenetics: hardware enabling neural control by light

  • Versatile, but Manually-Assembled, Optical Fiber/Laser System for In Vivo Optogenetics

  • Very Simple Off-The-Shelf Systems for In Vivo Optogenetics

Understanding normal and pathological brain computations

  • Closed-loop, ultraprecise, automated craniotomies

Home » Resources » Optogenetics: molecules enabling neural control by light » Jaws: red light-drivable halorhodopsin

Jaws: red light-drivable halorhodopsin

Publication [Chuong, A. S., Miri, M. L.*, Busskamp, V.*, Matthews, G.A.C.*, Acker, L.C.*, Soresnsen, A.T., Young, A., Klapoetke, N. C., Henninger, M.A., Kodandaramaiah, S.B., Ogawa, M., Ramanlal, S. B., Bandler, R. C., Allen, B. D., Forest, C.R., Chow, B.Y., Han, X., Lin, Y., Tye, K.M., Roska, B., Cardin, J.A., Boyden, E. S. (2014) Noninvasive optical inhibition with a red-shifted microbial rhodopsin, Nature Neuroscience 17:1123-1129. (*, equal contribution)]

Plasmids, plasmid maps, and plasmid preparation protocols, as well as viruses (AAV, lentivirus), available at [Addgene]

Viruses (AAV, lentivirus) available at [UNC vector core]

Viruses (AAV, lentivirus) available at [U. Penn vector core]

Transgenic mice (Cre-dependent) available at [
Jax] [Publication]

Contact [Prof. Ed Boyden] with questions, comments, or to check for updates.

Projects

Optogenetics: molecules enabling neural control by light

Copyright 2006-present, Ed Boyden | Accessibility